Our hosting provider will be performing UPS maintenance on Tuesday, Oct 25, 2016 between 8 AM and 5 PM PDT. SciCrunch searching services will be down during this time.

Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Ubiquitin-dependent and ubiquitin-independent control of subunit stoichiometry in the SWI/SNF complex.


The mammalian SWI/SNF chromatin remodeling complex is a key player in multiple chromatin transactions. Core subunits of this complex, including the ATPase, Brg-1, and various Brg-1-associated factors (BAFs), work in concert to maintain a functional remodeling complex. This intra-complex regulation is supervised by protein-protein interactions, as stoichiometric levels of BAF proteins are maintained by proteasomal degradation. We show that the mechanism of BAF155-mediated stabilization of BAF57 involves blocking its ubiquitination by preventing interaction with TRIP12, an E3 ubiquitin ligase. Consequently, as opposed to complexed BAF57, whose principal lysines are unavailable for ubiquitination, uncomplexed BAF57 can be freely ubiquitinated and degraded by the proteasome. Additionally, a BAF57 mutant, which contains no lysine residues, was found to retain its ability to be stabilized by interaction with BAF155, suggesting that in addition to the ubiquitin-dependent mechanism of BAF57 degradation, there exists a ubiquitin-independent mechanism that may involve the direct interaction of BAF57 with the proteasome. We propose that this regulatory mechanism exists to ensure functional fidelity of the complex and prevent the accumulation of uncomplexed proteins, which may disrupt the normal activity of the complex.

Pubmed ID: 20829358


  • Keppler BR
  • Archer TK


The Journal of biological chemistry

Publication Data

November 12, 2010

Associated Grants

  • Agency: NIEHS NIH HHS, Id: Z01 ES071006-10
  • Agency: Intramural NIH HHS, Id:

Mesh Terms

  • Amino Acid Substitution
  • Blotting, Western
  • Carrier Proteins
  • Cell Line, Tumor
  • Chromosomal Proteins, Non-Histone
  • DNA-Binding Proteins
  • Humans
  • Immunoprecipitation
  • Mutation
  • Proteasome Endopeptidase Complex
  • Protein Binding
  • Protein Subunits
  • RNA Interference
  • Transcription Factors
  • Transfection
  • Ubiquitin
  • Ubiquitin-Protein Ligases
  • Ubiquitination