• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Mediator and cohesin connect gene expression and chromatin architecture.

Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediator, a transcriptional coactivator, forms a complex with cohesin, which can form rings that connect two DNA segments. The cohesin-loading factor Nipbl is associated with mediator-cohesin complexes, providing a means to load cohesin at promoters. DNA looping is observed between the enhancers and promoters occupied by mediator and cohesin. Mediator and cohesin co-occupy different promoters in different cells, thus generating cell-type-specific DNA loops linked to the gene expression program of each cell.

Pubmed ID: 20720539

Authors

  • Kagey MH
  • Newman JJ
  • Bilodeau S
  • Zhan Y
  • Orlando DA
  • van Berkum NL
  • Ebmeier CC
  • Goossens J
  • Rahl PB
  • Levine SS
  • Taatjes DJ
  • Dekker J
  • Young RA

Journal

Nature

Publication Data

September 23, 2010

Associated Grants

  • Agency: NHGRI NIH HHS, Id: HG002668
  • Agency: NHGRI NIH HHS, Id: HG003143
  • Agency: NHGRI NIH HHS, Id: R01 HG002668
  • Agency: NHGRI NIH HHS, Id: R01 HG002668-07
  • Agency: NHGRI NIH HHS, Id: R01 HG003143
  • Agency: NHGRI NIH HHS, Id: R01 HG003143-06
  • Agency: Canadian Institutes of Health Research, Id:

Mesh Terms

  • Animals
  • Cell Cycle Proteins
  • Cells, Cultured
  • Chromatin
  • Chromatin Assembly and Disassembly
  • Chromosomal Proteins, Non-Histone
  • DNA
  • Embryonic Stem Cells
  • Enhancer Elements, Genetic
  • Fibroblasts
  • Gene Expression Regulation
  • Mediator Complex
  • Mice
  • Nucleic Acid Conformation
  • Organ Specificity
  • Promoter Regions, Genetic
  • Protein Binding