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Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.

eIF4E-binding proteins (4E-BPs) regulate translation of mRNAs in eukaryotes. However the extent to which specific mRNA targets are regulated by 4E-BPs remains unknown. We performed translational profiling by microarray analysis of polysome and monosome associated mRNAs in wild-type and mutant cells to identify mRNAs in yeast regulated by the 4E-BPs Caf20p and Eap1p; the first-global comparison of 4E-BP target mRNAs. We find that yeast 4E-BPs modulate the translation of >1000 genes. Most target mRNAs differ between the 4E-BPs revealing mRNA specificity for translational control by each 4E-BP. This is supported by observations that eap1Δ and caf20Δ cells have different nitrogen source utilization defects, implying different mRNA targets. To account for the mRNA specificity shown by each 4E-BP, we found correlations between our data sets and previously determined targets of yeast mRNA-binding proteins. We used affinity chromatography experiments to uncover specific RNA-stabilized complexes formed between Caf20p and Puf4p/Puf5p and between Eap1p and Puf1p/Puf2p. Thus the combined action of each 4E-BP with specific 3'-UTR-binding proteins mediates mRNA-specific translational control in yeast, showing that this form of translational control is more widely employed than previously thought.

Pubmed ID: 20705650


  • Cridge AG
  • Castelli LM
  • Smirnova JB
  • Selley JN
  • Rowe W
  • Hubbard SJ
  • McCarthy JE
  • Ashe MP
  • Grant CM
  • Pavitt GD


Nucleic acids research

Publication Data

December 14, 2010

Associated Grants

  • Agency: Biotechnology and Biological Sciences Research Council, Id: BB/G012571/1
  • Agency: Biotechnology and Biological Sciences Research Council, Id: BBD000106
  • Agency: Biotechnology and Biological Sciences Research Council, Id: BBG012571
  • Agency: Biotechnology and Biological Sciences Research Council, Id: G17520

Mesh Terms

  • Amino Acids
  • Cyclins
  • Eukaryotic Initiation Factors
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Nitrogen
  • Oligonucleotide Array Sequence Analysis
  • Protein Biosynthesis
  • RNA, Messenger
  • RNA-Binding Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Transcription, Genetic