Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

CaMKII triggers the diffusional trapping of surface AMPARs through phosphorylation of stargazin.

Neuron | Jul 29, 2010

The Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during both development and plasticity. However, the underlying mechanism is unknown. Using single-particle tracking of AMPARs, we show that CaMKII activation and postsynaptic translocation induce the synaptic trapping of AMPARs diffusing in the membrane. AMPAR immobilization requires both phosphorylation of the auxiliary subunit Stargazin and its binding to PDZ domain scaffolds. It does not depend on the PDZ binding domain of GluA1 AMPAR subunit nor its phosphorylation at Ser831. Finally, CaMKII-dependent AMPAR immobilization regulates short-term plasticity. Thus, NMDA-dependent Ca(2+) influx in the post-synapse triggers a CaMKII- and Stargazin-dependent decrease in AMPAR diffusional exchange at synapses that controls synaptic function.

Pubmed ID: 20670832 RIS Download

Mesh terms: Action Potentials | Animals | Animals, Newborn | Benzothiadiazines | Benzylamines | Calcium | Calcium Channels | Calcium-Calmodulin-Dependent Protein Kinase Type 2 | Cells, Cultured | Diffusion | Electric Stimulation | Embryo, Mammalian | Enzyme Activation | Excitatory Postsynaptic Potentials | Green Fluorescent Proteins | Hippocampus | Intracellular Signaling Peptides and Proteins | Membrane Proteins | Models, Biological | Neurons | Phosphorylation | Protein Kinase Inhibitors | Protein Transport | Rats | Rats, Sprague-Dawley | Receptors, AMPA | Statistics, Nonparametric | Sulfonamides | Transfection

Research resources used in this publication

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Canadian Institutes of Health Research, Id:

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

This is a list of tools and resources that we have found mentioned in this publication.