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We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Pubmed ID: 20639868 RIS Download
Mesh terms: Animals | Automation | Axons | Embryo, Nonmammalian | Equipment Design | Laser Therapy | Mass Screening | Microscopy, Confocal | Models, Animal | Mutation | Retina | Zebrafish
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