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High-throughput in vivo vertebrate screening.

We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

Pubmed ID: 20639868


  • Pardo-Martin C
  • Chang TY
  • Koo BK
  • Gilleland CL
  • Wasserman SC
  • Yanik MF


Nature methods

Publication Data

August 2, 2010

Associated Grants

  • Agency: NIH HHS, Id: 1-DP2-OD002989-01
  • Agency: NIH HHS, Id: DP2 OD002989
  • Agency: NIH HHS, Id: DP2 OD002989-01
  • Agency: NINDS NIH HHS, Id: R01 NS073127

Mesh Terms

  • Animals
  • Automation
  • Axons
  • Embryo, Nonmammalian
  • Equipment Design
  • Laser Therapy
  • Mass Screening
  • Microscopy, Confocal
  • Models, Animal
  • Mutation
  • Retina
  • Zebrafish