Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Gamete recognition in mice depends on the cleavage status of an egg's zona pellucida protein.

Science (New York, N.Y.) | Jul 9, 2010

At fertilization, mouse sperm bind to the zona pellucida (which consists of glycoproteins ZP1, ZP2, and ZP3) that surrounds eggs. A ZP2 cleavage model of gamete recognition requires intact ZP2, and a glycan release model postulates that zona glycans are ligands for sperm. These two models were tested by replacing endogenous protein with ZP2 that cannot be cleaved (Zp2(Mut)) or with ZP3 lacking implicated O glycans (Zp3(Mut)). Sperm bound to two-cell Zp2(Mut) embryos despite fertilization and cortical granule exocytosis. Contrary to prediction, sperm fertilized Zp3(Mut) eggs. Sperm at the surface of the zona pellucida remained acrosome-intact for more than 2 hours and were displaced by additional sperm. These data indicate that sperm-egg recognition depends on the cleavage status of ZP2 and that binding at the surface of the zona is not sufficient to induce sperm acrosome exocytosis.

Pubmed ID: 20616279 RIS Download

Mesh terms: Acrosome | Acrosome Reaction | Animals | Cell Adhesion | Egg Proteins | Embryo, Mammalian | Exocytosis | Female | Fertility | Fertilization | Ligands | Male | Membrane Glycoproteins | Mice | Mice, Transgenic | Models, Biological | Mutant Proteins | Polysaccharides | Receptors, Cell Surface | Sperm Capacitation | Sperm-Ovum Interactions | Spermatozoa | Zona Pellucida | Zona Pellucida Glycoproteins | Zygote