Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

CCM3 signaling through sterile 20-like kinases plays an essential role during zebrafish cardiovascular development and cerebral cavernous malformations.

Cerebral cavernous malformation is a common human vascular disease that arises due to loss-of-function mutations in genes encoding three intracellular adaptor proteins, cerebral cavernous malformations 1 protein (CCM1), CCM2, and CCM3. CCM1, CCM2, and CCM3 interact biochemically in a pathway required in endothelial cells during cardiovascular development in mice and zebrafish. The downstream effectors by which this signaling pathway regulates endothelial function have not yet been identified. Here we have shown in zebrafish that expression of mutant ccm3 proteins (ccm3Delta) known to cause cerebral cavernous malformation in humans confers cardiovascular phenotypes identical to those associated with loss of ccm1 and ccm2. CCM3Delta proteins interacted with CCM1 and CCM2, but not with other proteins known to bind wild-type CCM3, serine/threonine protein kinase MST4 (MST4), sterile 20-like serine/threonine kinase 24 (STK24), and STK25, all of which have poorly defined biological functions. Cardiovascular phenotypes characteristic of CCM deficiency arose due to stk deficiency and combined low-level deficiency of stks and ccm3 in zebrafish embryos. In cultured human endothelial cells, CCM3 and STK25 regulated barrier function in a manner similar to CCM2, and STKs negatively regulated Rho by directly activating moesin. These studies identify STKs as essential downstream effectors of CCM signaling in development and disease that may regulate both endothelial and epithelial cell junctions.

Pubmed ID: 20592472


  • Zheng X
  • Xu C
  • Di Lorenzo A
  • Kleaveland B
  • Zou Z
  • Seiler C
  • Chen M
  • Cheng L
  • Xiao J
  • He J
  • Pack MA
  • Sessa WC
  • Kahn ML


The Journal of clinical investigation

Publication Data

August 3, 2010

Associated Grants

  • Agency: NHLBI NIH HHS, Id: R01 HL094326
  • Agency: NHLBI NIH HHS, Id: R01 HL094326
  • Agency: NHLBI NIH HHS, Id: R01 HL102138
  • Agency: NHLBI NIH HHS, Id: T32 HL07971

Mesh Terms

  • Amino Acid Sequence
  • Animals
  • Apoptosis Regulatory Proteins
  • Cardiovascular System
  • Conserved Sequence
  • Hemangioma, Cavernous, Central Nervous System
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Microfilament Proteins
  • Microtubule-Associated Proteins
  • Molecular Sequence Data
  • Phosphorylation
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins
  • Sequence Alignment
  • Signal Transduction
  • Zebrafish
  • Zebrafish Proteins