Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

MOV10L1 is necessary for protection of spermatocytes against retrotransposons by Piwi-interacting RNAs.

http://www.ncbi.nlm.nih.gov/pubmed/20547853

Piwi-interacting RNAs (piRNAs) comprise a broad class of small noncoding RNAs that function as an endogenous defense system against transposable elements. Here we show that the putative DExD-box helicase MOV10-like-1 (MOV10L1) is essential for silencing retrotransposons in the mouse male germline. Mov10l1 is specifically expressed in germ cells with increasing expression from gonocytes/type A spermatogonia to pachytene spermatocytes. Primary spermatocytes of Mov10l1(-/-) mice show activation of LTR and LINE-1 retrotransposons, followed by cell death, causing male infertility and a complete block of spermatogenesis at early prophase of meiosis I. Despite the early expression of Mov10l1, germline stem cell maintenance appears unaffected in Mov10l1(-/-) mice. MOV10L1 interacts with the Piwi proteins MILI and MIWI. MOV10L1 also interacts with heat shock 70-kDa protein 2 (HSPA2), a testis-enriched chaperone expressed in pachytene spermatocytes and also essential for male fertility. These studies reveal a crucial role of MOV10L1 in male fertility and piRNA-directed retrotransposon silencing in male germ cells and suggest that MOV10L1 functions as a key component of a safeguard mechanism for the genetic information in male germ cells of mammals.

Pubmed ID: 20547853 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Argonaute Proteins | Base Sequence | DNA Methylation | DNA Primers | Fertility | HSP70 Heat-Shock Proteins | Male | Meiosis | Mice | Mice, Knockout | Models, Biological | Molecular Sequence Data | Mutation | Proteins | RNA Helicases | RNA, Small Interfering | Retroelements | Sequence Homology, Amino Acid | Spermatocytes | Spermatogenesis | Testis

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NICHD NIH HHS, Id: R01 HD036022
  • Agency: NICHD NIH HHS, Id: R01 HD036022-09
  • Agency: NICHD NIH HHS, Id: R01 HD036022-10
  • Agency: NICHD NIH HHS, Id: R01 HD053889
  • Agency: NICHD NIH HHS, Id: R01 HD061575
  • Agency: NHLBI NIH HHS, Id: R01 HL093039

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.