Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Hip2 interacts with and destabilizes Smac/DIABLO.

http://www.ncbi.nlm.nih.gov/pubmed/20537984

Hip2 is a ubiquitin-conjugating enzyme that is involved in the cell cycle and suppression of cell death. To understand its role further, we tried to identify proteins that interact with Hip2. Using the immunoprecipitation technique and one-dimensional gel electrophoresis, we identified Smac/DIABLO, a proapoptotic molecule, as a protein that interacts with Hip2. The interaction of Hip2 and Smac was confirmed through in vivo and in vitro experiments. Hip2 promoted degradation of mature Smac through the ubiquitin proteasome pathway. As a result, Hip2 significantly blocked cell death induced by staurosporine and Smac. This study suggests that Hip2 might be involved in the regulation of Smac-mediated apoptosis.

Pubmed ID: 20537984 RIS Download

Mesh terms: Apoptosis | Cell Line | Humans | Immunoprecipitation | Intracellular Signaling Peptides and Proteins | Mitochondrial Proteins | Proteasome Endopeptidase Complex | Protein Stability | Ubiquitin-Conjugating Enzymes | Ubiquitination

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.