Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Break-induced replication requires all essential DNA replication factors except those specific for pre-RC assembly.

Genes & development | Jun 1, 2010

http://www.ncbi.nlm.nih.gov/pubmed/20516198

Break-induced replication (BIR) is an efficient homologous recombination (HR) pathway employed to repair a DNA double-strand break (DSB) when homology is restricted to one end. All three major replicative DNA polymerases are required for BIR, including the otherwise nonessential Pol32 subunit. Here we show that BIR requires the replicative DNA helicase (Cdc45, the GINS, and Mcm2-7 proteins) as well as Cdt1. In contrast, both subunits of origin recognition complex (ORC) and Cdc6, which are required to create a prereplication complex (pre-RC), are dispensable. The Cdc7 kinase, required for both initiation of DNA replication and post-replication repair (PRR), is also required for BIR. Ubiquitination and sumoylation of the DNA processivity clamp PCNA play modest roles; in contrast, PCNA alleles that suppress pol32Delta's cold sensitivity fail to suppress its role in BIR, and are by themselves dominant inhibitors of BIR. These results suggest that origin-independent BIR involves cross-talk between normal DNA replication factors and PRR.

Pubmed ID: 20516198 RIS Download

Mesh terms: Alleles | Cell Cycle Proteins | Cold Temperature | DNA Breaks, Double-Stranded | DNA Helicases | DNA Repair | DNA Replication | DNA-Binding Proteins | Mutation | Origin Recognition Complex | Protein-Serine-Threonine Kinases | SUMO-1 Protein | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Ubiquitination

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM07122
  • Agency: NIGMS NIH HHS, Id: GM20056
  • Agency: NIGMS NIH HHS, Id: GM32431
  • Agency: NIGMS NIH HHS, Id: GM76020
  • Agency: NIGMS NIH HHS, Id: R01 GM032431
  • Agency: NIGMS NIH HHS, Id: R01 GM045436
  • Agency: NIGMS NIH HHS, Id: R01 GM076020
  • Agency: NIGMS NIH HHS, Id: T32 GM007122

GO (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.