• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Enhanced neuronal RNAi in C. elegans using SID-1.

We expressed SID-1, a transmembrane protein from Caenorhabditis elegans that is required for systemic RNA interference (RNAi), in C. elegans neurons. This expression increased the response of neurons to double-stranded (ds)RNA delivered by feeding. Mutations in the lin-15b and lin-35 genes enhanced this effect. Worms expressing neuronal SID-1 showed RNAi phenotypes when fed with bacteria expressing dsRNA for known neuronal genes and for uncharacterized genes with no previously known neuronal phenotypes. Neuronal expression of sid-1 decreased nonneuronal RNAi, suggesting that neurons expressing transgenic sid-1(+) served as a sink for dsRNA. This effect, or a sid-1(-) background, can be used to uncover neuronal defects for lethal genes. Expression of sid-1(+) from cell-specific promoters in sid-1 mutants results in cell-specific feeding RNAi. We used these strains to identify a role for integrin signaling genes in mechanosensation.

Pubmed ID: 20512143

Authors

  • Calixto A
  • Chelur D
  • Topalidou I
  • Chen X
  • Chalfie M

Journal

Nature methods

Publication Data

July 30, 2010

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM30997
  • Agency: NIGMS NIH HHS, Id: R37 GM030997
  • Agency: NIGMS NIH HHS, Id: R37 GM030997-25
  • Agency: NIGMS NIH HHS, Id: R37 GM030997-26
  • Agency: NIGMS NIH HHS, Id: R37 GM030997-27
  • Agency: NIGMS NIH HHS, Id: R37 GM030997-27S1

Mesh Terms

  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins
  • Gene Expression Regulation
  • Genes, Lethal
  • Membrane Proteins
  • Neurons
  • RNA Interference
  • Repressor Proteins
  • Transcription Factors