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A global protein kinase and phosphatase interaction network in yeast.

The interactions of protein kinases and phosphatases with their regulatory subunits and substrates underpin cellular regulation. We identified a kinase and phosphatase interaction (KPI) network of 1844 interactions in budding yeast by mass spectrometric analysis of protein complexes. The KPI network contained many dense local regions of interactions that suggested new functions. Notably, the cell cycle phosphatase Cdc14 associated with multiple kinases that revealed roles for Cdc14 in mitogen-activated protein kinase signaling, the DNA damage response, and metabolism, whereas interactions of the target of rapamycin complex 1 (TORC1) uncovered new effector kinases in nitrogen and carbon metabolism. An extensive backbone of kinase-kinase interactions cross-connects the proteome and may serve to coordinate diverse cellular responses.

Pubmed ID: 20489023

Authors

  • Breitkreutz A
  • Choi H
  • Sharom JR
  • Boucher L
  • Neduva V
  • Larsen B
  • Lin ZY
  • Breitkreutz BJ
  • Stark C
  • Liu G
  • Ahn J
  • Dewar-Darch D
  • Reguly T
  • Tang X
  • Almeida R
  • Qin ZS
  • Pawson T
  • Gingras AC
  • Nesvizhskii AI
  • Tyers M

Journal

Science (New York, N.Y.)

Publication Data

May 21, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: CA-126239
  • Agency: Canadian Institutes of Health Research, Id: MOP-12246
  • Agency: Canadian Institutes of Health Research, Id: MOP-57793
  • Agency: Canadian Institutes of Health Research, Id: MOP-84314
  • Agency: NCI NIH HHS, Id: R01 CA126239
  • Agency: NIGMS NIH HHS, Id: R01 GM094231
  • Agency: NIH HHS, Id: R01 OD010929
  • Agency: NCRR NIH HHS, Id: R01 RR024031
  • Agency: NCRR NIH HHS, Id: R01 RR024031-05
  • Agency: NCRR NIH HHS, Id: R01RR024031

Mesh Terms

  • Binding Sites
  • Carbon
  • Cell Cycle Proteins
  • DNA Damage
  • MAP Kinase Signaling System
  • Mass Spectrometry
  • Metabolic Networks and Pathways
  • Models, Biological
  • Nitrogen
  • Phosphoprotein Phosphatases
  • Phosphorylation
  • Protein Interaction Mapping
  • Protein Kinases
  • Protein Subunits
  • Protein Tyrosine Phosphatases
  • Protein-Serine-Threonine Kinases
  • Proteome
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Signal Transduction