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An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.

The karyopherin CRM1 mediates nuclear export of proteins and ribonucleoproteins bearing a leucine-rich nuclear export signal (NES). To elucidate the precise mechanism by which NES-cargos are dissociated from CRM1 in the cytoplasm, which is important for transport directionality, we determined a 2.0-A resolution crystal structure of yeast CRM1:RanBP1:RanGTP complex, an intermediate in the disassembly of the CRM1 nuclear export complex. The structure shows that on association of Ran-binding domain (RanBD) of RanBP1 with CRM1:NES-cargo:RanGTP complex, RanBD and the C-terminal acidic tail of Ran induce a large movement of the intra-HEAT9 loop of CRM1. The loop moves to the CRM1 inner surface immediately behind the NES-binding site and causes conformational rearrangements in HEAT repeats 11 and 12 so that the hydrophobic NES-binding cleft on the CRM1 outer surface closes, squeezing out the NES-cargo. This allosteric mechanism accelerates dissociation of NES by over two orders of magnitude. Structure-based mutagenesis indicated that the HEAT9 loop also functions as an allosteric autoinhibitor to stabilize CRM1 in a conformation that is unable to bind NES-cargo in the absence of RanGTP.

Pubmed ID: 20485264


  • Koyama M
  • Matsuura Y


The EMBO journal

Publication Data

June 16, 2010

Associated Grants


Mesh Terms

  • Active Transport, Cell Nucleus
  • Allosteric Regulation
  • Crystallography, X-Ray
  • Guanosine Triphosphate
  • Karyopherins
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Nuclear Proteins
  • Protein Binding
  • Protein Structure, Quaternary
  • Receptors, Cytoplasmic and Nuclear
  • Saccharomyces cerevisiae