The miR-143-adducin3 pathway is essential for cardiac chamber morphogenesis.
Discovering the genetic and cellular mechanisms that drive cardiac morphogenesis remains a fundamental goal, as three-dimensional architecture greatly impacts functional capacity. During development, accurately contoured chambers balloon from a primitive tube in a process characterized by regional changes in myocardial cell size and shape. How these localized changes are achieved remains elusive. Here, we show in zebrafish that microRNA-143 (miR-143) is required for chamber morphogenesis through direct repression of adducin3 (add3), which encodes an F-actin capping protein. Knockdown of miR-143 or disruption of the miR-143-add3 interaction inhibits ventricular cardiomyocyte F-actin remodeling, which blocks their normal growth and elongation and leads to ventricular collapse and decreased contractility. Using mosaic analyses, we find that miR-143 and add3 act cell-autonomously to control F-actin dynamics and cell morphology. As proper chamber emergence relies on precise control of cytoskeletal polymerization, Add3 represents an attractive target to be fine-tuned by both uniform signals, such as miR-143, and undiscovered localized signals. Together, our data uncover the miR-143-add3 genetic pathway as essential for cardiac chamber formation and function through active adjustment of myocardial cell morphology.
Pubmed ID: 20460367 RIS Download
3' Untranslated Regions | Actins | Animals | Base Sequence | Calmodulin-Binding Proteins | Gene Expression Regulation, Developmental | Heart | In Situ Hybridization | MicroRNAs | Myocytes, Cardiac | Oligodeoxyribonucleotides, Antisense | Sequence Homology, Nucleic Acid | Zebrafish | Zebrafish Proteins