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PHF8 targets histone methylation and RNA polymerase II to activate transcription.

Mutations in PHF8 are associated with X-linked mental retardation and cleft lip/cleft palate. PHF8 contains a plant homeodomain (PHD) in its N terminus and is a member of a family of JmjC domain-containing proteins. While PHDs can act as methyl lysine recognition motifs, JmjC domains can catalyze lysine demethylation. Here, we show that PHF8 is a histone demethylase that removes repressive histone H3 dimethyl lysine 9 marks. Our biochemical analysis revealed specific association of the PHF8 PHD with histone H3 trimethylated at lysine 4 (H3K4me3). Chromatin immunoprecipitation followed by high-throughput sequencing indicated that PHF8 is enriched at the transcription start sites of many active or poised genes, mirroring the presence of RNA polymerase II (RNAPII) and of H3K4me3-bearing nucleosomes. We show that PHF8 can act as a transcriptional coactivator and that its activation function largely depends on binding of the PHD to H3K4me3. Furthermore, we present evidence for direct interaction of PHF8 with the C-terminal domain of RNAPII. Importantly, a PHF8 disease mutant was defective in demethylation and in coactivation. This is the first demonstration of a chromatin-modifying enzyme that is globally recruited to promoters through its association with H3K4me3 and RNAPII.

Pubmed ID: 20421419


  • Fortschegger K
  • de Graaf P
  • Outchkourov NS
  • van Schaik FM
  • Timmers HT
  • Shiekhattar R


Molecular and cellular biology

Publication Data

July 11, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: CA090758
  • Agency: Austrian Science Fund FWF, Id: J 2728-B12

Mesh Terms

  • Amino Acid Sequence
  • Cell Line
  • Gene Expression Profiling
  • Gene Knockdown Techniques
  • Histone Demethylases
  • Histones
  • Humans
  • Lysine
  • Methylation
  • Microarray Analysis
  • Molecular Sequence Data
  • RNA Polymerase II
  • Recombinant Fusion Proteins
  • Transcription Factors