We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Cooperation between translating ribosomes and RNA polymerase in transcription elongation.

Science (New York, N.Y.) | Apr 23, 2010

During transcription of protein-coding genes, bacterial RNA polymerase (RNAP) is closely followed by a ribosome that translates the newly synthesized transcript. Our in vivo measurements show that the overall elongation rate of transcription is tightly controlled by the rate of translation. Acceleration and deceleration of a ribosome result in corresponding changes in the speed of RNAP. Moreover, we found an inverse correlation between the number of rare codons in a gene, which delay ribosome progression, and the rate of transcription. The stimulating effect of a ribosome on RNAP is achieved by preventing its spontaneous backtracking, which enhances the pace and also facilitates readthrough of roadblocks in vivo. Such a cooperative mechanism ensures that the transcriptional yield is always adjusted to translational needs at different genes and under various growth conditions.

Pubmed ID: 20413502 RIS Download

Mesh terms: Anti-Bacterial Agents | Chloramphenicol | Codon | DNA-Directed RNA Polymerases | Escherichia coli | Escherichia coli Proteins | Genes, Bacterial | Lac Operon | Peptide Chain Elongation, Translational | Protein Biosynthesis | RNA, Bacterial | RNA, Messenger | Ribosomes | Streptomycin | Transcription, Genetic | beta-Galactosidase

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM058750
  • Agency: NIGMS NIH HHS, Id: R01 GM058750-12

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.