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Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little information on the function of individual 60S ribosomal proteins in ribosome synthesis. Herein, we have analysed the contribution of ribosomal protein L35 in ribosome biogenesis. In vivo depletion of L35 results in a deficit in 60S ribosomal subunits and the appearance of half-mer polysomes. Pulse-chase, northern hybridization and primer extension analyses show that processing of the 27SB to 7S pre-rRNAs is strongly delayed upon L35 depletion. Most likely as a consequence of this, release of pre-60S ribosomal particles from the nucleolus to the nucleoplasm is also blocked. Deletion of RPL35A leads to similar although less pronounced phenotypes. Moreover, we show that L35 assembles in the nucleolus and binds to early pre-60S ribosomal particles. Finally, flow cytometry analysis indicated that L35-depleted cells mildly delay the G1 phase of the cell cycle. We conclude that L35 assembly is a prerequisite for the efficient cleavage of the internal transcribed spacer 2 at site C(2).
Pubmed ID: 20392820 RIS Download
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A program of regional geochemical and geophysical surveys designed to attract the mineral exploration industry to an under-explored region of British Columbia between Williams Lake and Mackenize. The QUEST Project is focused on the Quesnel Terrane, which has good potential for copper and gold porphyry deposits, but in this region is covered by a thick layer of sand and gravel left behind by glaciers. Geoscience BC's QUEST Project included two airborne geophysical surveys: an airborne electromagnetic (EM) survey and an airborne gravity survey, which give insight into the conductivity and density of the rocks. The results of the two airborne surveys are an important tool for industry looking to understand the geology in the QUEST Project area. The QUEST Project also included the reanalysis of almost 5000 archived regional geochemical samples from parts of NTS sheets 93A, B, G, H, K and N, and the collection of 2200 new geochemical samples north and west of Prince George. This new geochemical data is helping industry to identify new exploration targets. Subsequent follow-up work has included analysis and inversion of datasets and updating the regional bedrock geology map.
View all literature mentionsNon profit, private research and education institution that performs molecular and genetic research used to generate methods for better diagnostics and treatments for cancer and neurological diseases. Research of cancer causing genes and their respective signaling pathways, mutations and structural variations of the human genome that could cause neurodevelopmental and neurodegenerative illnesses such as autism, schizophrenia, and Alzheimer's and Parkinson's diseases and also research in plant genetics and quantitative biology.
View all literature mentionsA curated database that provides comprehensive integrated biological information for Saccharomyces cerevisiae along with search and analysis tools to explore these data. SGD allows researchers to discover functional relationships between sequence and gene products in fungi and higher organisms. The SGD also maintains the S. cerevisiae Gene Name Registry, a complete list of all gene names used in S. cerevisiae which includes a set of general guidelines to gene naming. Protein Page provides basic protein information calculated from the predicted sequence and contains links to a variety of secondary structure and tertiary structure resources. Yeast Biochemical Pathways allows users to view and search for biochemical reactions and pathways that occur in S. cerevisiae as well as map expression data onto the biochemical pathways. Literature citations are provided where available.
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