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Esophageal squamous cell dysplasia and delayed differentiation with deletion of krüppel-like factor 4 in murine esophagus.

BACKGROUND & AIMS: Krüppel-like factor 4 (Klf; previously known a gut-enriched Krüppel-like factor) is a DNA-binding transcriptional regulator highly expressed in skin and gastrointestinal epithelia, specifically in regions of cellular differentiation. Homozygous null mice for Klf4 die shortly after birth from skin defects, precluding their analysis at later stages. The aim of this study was to analyze the function of Klf4 in keratinocyte biology and epithelial homeostasis in the adult by focusing on the squamous lined esophagus. METHODS: By using the ED-L2 promoter of Epstein-Barr virus to drive Cre, we obtained tissue-specific ablation of Klf4 in the squamous epithelia of the tongue, esophagus, and forestomach. RESULTS: Mice with loss of Klf4 in esophageal epithelia survived to adulthood, bypassing the early lethality. Tissue-specific Klf4 knockout mice had increased basal cell proliferation and a delay in cellular maturation; these mice developed epithelial hypertrophy and subsequent dysplasia by 6 months of age. Moreover, loss of Klf4 in vivo was associated with increased expression of the pro-proliferative Klf5, and Klf4 down-regulated Klf5 both transcriptionally and posttranscriptionally. By using gene expression profiling, we also showed decreased expression of critical late-stage differentiation factors and identified alterations of several genes important in cellular differentiation. CONCLUSIONS: Klf4 is essential for squamous epithelial differentiation in vivo and interacts with Klf5 to maintain normal epithelial homeostasis.

Pubmed ID: 20347813

Authors

  • Tetreault MP
  • Yang Y
  • Travis J
  • Yu QC
  • Klein-Szanto A
  • Tobias JW
  • Katz JP

Journal

Gastroenterology

Publication Data

July 12, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: P01 CA098101
  • Agency: NCI NIH HHS, Id: P30 CA016520
  • Agency: NIDDK NIH HHS, Id: P30 DK050306
  • Agency: NIDDK NIH HHS, Id: R01 DK069984
  • Agency: NIDDK NIH HHS, Id: R01 DK069984
  • Agency: NIDDK NIH HHS, Id: R01 DK069984-01A2

Mesh Terms

  • Animals
  • Apoptosis
  • Cell Differentiation
  • Cells, Cultured
  • Esophagus
  • Female
  • Herpesvirus 4, Human
  • Kruppel-Like Transcription Factors
  • Male
  • Mice
  • Mice, Knockout
  • Promoter Regions, Genetic
  • beta Catenin