Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Dact1 is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain.

Dact1 (Dapper/Frodo), an intracellular phosphoprotein that binds Dishevelled, catenins, and other signaling proteins, is expressed in the developing and mature mammalian CNS, but its function there is unknown. Dact1 colocalized with synaptic markers and partitioned to postsynaptic fractions from cultured mouse forebrain neurons. Hippocampal neurons from Dact1 knock-out mice had simpler dendritic arbors and fewer spines than hippocampal neurons from wild-type littermates. This correlated with reductions in excitatory synapses and miniature EPSCs, whereas inhibitory synapses were not affected. Loss of Dact1 resulted in a decrease in activated Rac, and recombinant expression of either Dact1 or constitutively active Rac, but not Rho or Cdc42, rescued dendrite and spine phenotypes in Dact1 mutant neurons. Our findings suggest that, during neuronal differentiation, Dact1 plays a critical role in a molecular pathway promoting Rac activity underlying the elaboration of dendrites and the establishment of spines and excitatory synapses.

Pubmed ID: 20335472 RIS Download

Mesh terms: Age Factors | Analysis of Variance | Animals | Animals, Newborn | Cells, Cultured | Dendritic Spines | Disks Large Homolog 4 Protein | Excitatory Postsynaptic Potentials | GABA Plasma Membrane Transport Proteins | Green Fluorescent Proteins | Guanylate Kinases | Hippocampus | Intracellular Signaling Peptides and Proteins | Membrane Proteins | Mice | Mice, Knockout | Neurons | Silver Staining | Subcellular Fractions | Synapses | Vesicular Glutamate Transport Protein 1 | rac GTP-Binding Proteins

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Autism Speaks, Id: AS4654
  • Agency: NIMH NIH HHS, Id: R21 MH085995
  • Agency: NIMH NIH HHS, Id: R21 MH085995-01
  • Agency: NIMH NIH HHS, Id: R21MH085995

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.