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The trafficking protein Tmed2/p24beta(1) is required for morphogenesis of the mouse embryo and placenta.

Developmental biology | May 1, 2010

http://www.ncbi.nlm.nih.gov/pubmed/20178780

During vesicular transport between the endoplasmic reticulum and the Golgi, members of the TMED/p24 protein family form hetero-oligomeric complexes that facilitate protein-cargo recognition as well as vesicle budding. In addition, they regulate each other's level of expression. Despite analyses of TMED/p24 protein distribution in mammalian cells, yeast, and C. elegans, little is known about the role of this family in vertebrate embryogenesis. We report the presence of a single point mutation in Tmed2/p24beta(1) in a mutant mouse line, 99J, identified in an ENU mutagenesis screen for recessive developmental abnormalities. This mutation does not affect Tmed2/p24beta(1) mRNA levels but results in loss of TMED2/p24beta(1) protein. Prior to death at mid-gestation, 99J homozygous mutant embryos exhibit developmental delay, abnormal rostral-caudal elongation, randomized heart looping, and absence of the labyrinth layer of the placenta. We find that Tmed2/p24beta(1) is normally expressed in tissues showing morphological defects in 99J mutant embryos and that these affected tissues lack the TMED2/p24beta(1) oligomerization partners, TMED7/p24gamma(3) and TMED10/p24delta(1). Our data reveal a requirement for TMED2/p24beta(1) protein in the morphogenesis of the mouse embryo and placenta.

Pubmed ID: 20178780 RIS Download

Mesh terms: Animals | Embryo, Mammalian | Endoplasmic Reticulum | Female | Gene Expression Regulation, Developmental | Mice | Mice, Inbred C57BL | Morphogenesis | Mutation | Placenta | Pregnancy | Vesicular Transport Proteins

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Associated grants

  • Agency: NICHD NIH HHS, Id: U01 HD043478
  • Agency: NICHD NIH HHS, Id: U01 HD043478-05

Mouse Genome Informatics (Data, Gene Annotation)

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