Are hosting provider is investigating network issues. We apologize for the inconvenience.
Are hosting provider is investigating network issues. We apologize for the inconvenience.
Are hosting provider is investigating network issues. We apologize for the inconvenience.
Are hosting provider is investigating network issues. We apologize for the inconvenience.
Are hosting provider is investigating network issues. We apologize for the inconvenience.

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

miR-9, a MYC/MYCN-activated microRNA, regulates E-cadherin and cancer metastasis.

Nature cell biology | Mar 1, 2010

MicroRNAs (miRNAs) are increasingly implicated in regulating the malignant progression of cancer. Here we show that miR-9, which is upregulated in breast cancer cells, directly targets CDH1, the E-cadherin-encoding messenger RNA, leading to increased cell motility and invasiveness. miR-9-mediated E-cadherin downregulation results in the activation of beta-catenin signalling, which contributes to upregulated expression of the gene encoding vascular endothelial growth factor (VEGF); this leads, in turn, to increased tumour angiogenesis. Overexpression of miR-9 in otherwise non-metastatic breast tumour cells enables these cells to form pulmonary micrometastases in mice. Conversely, inhibiting miR-9 by using a 'miRNA sponge' in highly malignant cells inhibits metastasis formation. Expression of miR-9 is activated by MYC and MYCN, both of which directly bind to the mir-9-3 locus. Significantly, in human cancers, miR-9 levels correlate with MYCN amplification, tumour grade and metastatic status. These findings uncover a regulatory and signalling pathway involving a metastasis-promoting miRNA that is predicted to directly target expression of the key metastasis-suppressing protein E-cadherin.

Pubmed ID: 20173740 RIS Download

Mesh terms: 3' Untranslated Regions | Animals | Breast Neoplasms | Cadherins | Cell Line | Cell Line, Tumor | Cell Proliferation | DNA | Down-Regulation | Epithelial Cells | Female | Gene Dosage | Gene Expression | Gene Expression Regulation, Neoplastic | Histones | Humans | Lung Neoplasms | Mice | Mice, Inbred BALB C | Mice, Inbred NOD | Mice, SCID | MicroRNAs | N-Myc Proto-Oncogene Protein | Neoplasm Invasiveness | Neoplasm Metastasis | Neoplasms | Neovascularization, Pathologic | Neuroblastoma | Nuclear Proteins | Oncogene Proteins | Protein Binding | Proto-Oncogene Proteins c-myc | RNA, Small Interfering | Signal Transduction | Transfection | Transplantation, Heterologous | Vascular Endothelial Growth Factor A | Vimentin | beta Catenin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: K99 CA138572
  • Agency: NCI NIH HHS, Id: K99 CA138572-01

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.