Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Essential role of Tip60-dependent recruitment of ribonucleotide reductase at DNA damage sites in DNA repair during G1 phase.

A balanced deoxyribonucleotide (dNTP) supply is essential for DNA repair. Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and RRM2 accumulated very rapidly at damage sites. RRM1 bound physically to Tip60. Chromatin immunoprecipitation analyses of cells with an I-SceI cassette revealed that RRM1 bound to a damage site in a Tip60-dependent manner. Active RRM1 mutants lacking Tip60 binding failed to rescue an impaired DNA repair in RRM1-depleted G1-phase cells. Inhibition of RNR recruitment by an RRM1 C-terminal fragment sensitized cells to DNA damage. We propose that Tip60-dependent recruitment of RNR plays an essential role in dNTP supply for DNA repair.

Pubmed ID: 20159953


  • Niida H
  • Katsuno Y
  • Sengoku M
  • Shimada M
  • Yukawa M
  • Ikura M
  • Ikura T
  • Kohno K
  • Shima H
  • Suzuki H
  • Tashiro S
  • Nakanishi M


Genes & development

Publication Data

February 15, 2010

Associated Grants


Mesh Terms

  • Animals
  • DNA Damage
  • G1 Phase
  • Gene Knockdown Techniques
  • HeLa Cells
  • Histone Acetyltransferases
  • Humans
  • Mice
  • Ribonucleotide Reductases
  • Trans-Activators