• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

BACH1/FANCJ acts with TopBP1 and participates early in DNA replication checkpoint control.

Human TopBP1 plays a critical role in the control of DNA replication checkpoint. In this study, we report a specific interaction between TopBP1 and BACH1/FANCJ, a DNA helicase involved in the repair of DNA crosslinks. The TopBP1/BACH1 interaction is mediated by the very C-terminal tandem BRCT domains of TopBP1 and S phase-specific phosphorylation of BACH1 at Thr 1133 site. Interestingly, we demonstrate that depletion of TopBP1 or BACH1 attenuates the loading of RPA on chromatin. Moreover, both TopBP1 and BACH1 are required for ATR-dependent phosphorylation events in response to replication stress. Taken together, our data suggest that BACH1 has an unexpected early role in replication checkpoint control. A specific interaction between TopBP1 and BACH1 is likely to be required for the extension of single-stranded DNA regions and RPA loading following replication stress, which is a prerequisite for the subsequent activation of replication checkpoint.

Pubmed ID: 20159562

Authors

  • Gong Z
  • Kim JE
  • Leung CC
  • Glover JN
  • Chen J

Journal

Molecular cell

Publication Data

February 12, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: CA089239
  • Agency: NCI NIH HHS, Id: CA092312
  • Agency: NCI NIH HHS, Id: CA100109
  • Agency: NCI NIH HHS, Id: P01 CA092584
  • Agency: NCI NIH HHS, Id: R01 CA089239
  • Agency: NCI NIH HHS, Id: R01 CA089239-10
  • Agency: NCI NIH HHS, Id: R01 CA089239-11
  • Agency: NCI NIH HHS, Id: R01 CA092312
  • Agency: NCI NIH HHS, Id: R01 CA092312-10
  • Agency: NCI NIH HHS, Id: R01 CA092312-11
  • Agency: NCI NIH HHS, Id: R01 CA100109
  • Agency: NCI NIH HHS, Id: R01 CA100109-08
  • Agency: NCI NIH HHS, Id: R01 CA100109-09

Mesh Terms

  • Ataxia Telangiectasia Mutated Proteins
  • Basic-Leucine Zipper Transcription Factors
  • Carrier Proteins
  • Cell Cycle Proteins
  • Cell Line
  • Chromatin
  • DNA Damage
  • DNA Replication
  • DNA-Binding Proteins
  • Fanconi Anemia Complementation Group Proteins
  • HeLa Cells
  • Humans
  • Models, Genetic
  • Nuclear Proteins
  • Phosphorylation
  • Protein-Serine-Threonine Kinases
  • Replication Protein A