PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.
Parkinson's disease is the most common neurodegenerative movement disorder. Mutations in PINK1 and PARKIN are the most frequent causes of recessive Parkinson's disease. However, their molecular contribution to pathogenesis remains unclear. Here, we reveal important mechanistic steps of a PINK1/Parkin-directed pathway linking mitochondrial damage, ubiquitylation and autophagy in non-neuronal and neuronal cells. PINK1 kinase activity and its mitochondrial localization sequence are prerequisites to induce translocation of the E3 ligase Parkin to depolarized mitochondria. Subsequently, Parkin mediates the formation of two distinct poly-ubiquitin chains, linked through Lys 63 and Lys 27. In addition, the autophagic adaptor p62/SQSTM1 is recruited to mitochondrial clusters and is essential for the clearance of mitochondria. Strikingly, we identified VDAC1 (voltage-dependent anion channel 1) as a target for Parkin-mediated Lys 27 poly-ubiquitylation and mitophagy. Moreover, pathogenic Parkin mutations interfere with distinct steps of mitochondrial translocation, ubiquitylation and/or final clearance through mitophagy. Thus, our data provide functional links between PINK1, Parkin and the selective autophagy of mitochondria, which is implicated in the pathogenesis of Parkinson's disease.
Pubmed ID: 20098416
- Geisler S
- Holmström KM
- Skujat D
- Fiesel FC
- Rothfuss OC
- Kahle PJ
- Springer W
Nature cell biology
February 1, 2010
- Adaptor Proteins, Signal Transducing
- Biological Transport
- Cell Line, Tumor
- Fluorescent Antibody Technique
- HeLa Cells
- Parkinson Disease
- Protein Kinases
- RNA Interference
- Ubiquitin-Protein Ligases
- Voltage-Dependent Anion Channel 1
- Adenocarcinoma of lung, somatic is related to genes PRKN, PARK2, PDJ, LPRS2 which are autosomal recessive according to the OMIM database.
- Adenocarcinoma, ovarian, somatic is related to genes PRKN, PARK2, PDJ, LPRS2 which are autosomal dominant according to the OMIM database.
- Leprosy, susceptibility to is related to genes PRKN, PARK2, PDJ, LPRS2.
- Parkinson disease, juvenile, type 2 is related to genes PRKN, PARK2, PDJ, LPRS2 which are autosomal recessive according to the OMIM database.
- Parkinson disease 6, early onset is related to genes PINK1, PARK6 which are autosomal recessive according to the OMIM database.