• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Revelation of p53-independent function of MTA1 in DNA damage response via modulation of the p21 WAF1-proliferating cell nuclear antigen pathway.

Although metastasis-associated protein 1 (MTA1), a component of the nucleosome remodeling and deacetylase (NuRD) complex, is a DNA-damage response protein and regulates p53-dependent DNA repair, it remains unknown whether MTA1 also participates in p53-independent DNA damage response. Here, we provide evidence that MTA1 is a p53-independent transcriptional corepressor of p21(WAF1), and the underlying mechanism involves recruitment of MTA1-histone deacetylase 2 (HDAC2) complexes onto two selective regions of the p21(WAF1) promoter. Accordingly, MTA1 depletion, despite its effect on p53 down-regulation, superinduces p21(WAF1), increases p21(WAF1) binding to proliferating cell nuclear antigen (PCNA), and decreases the nuclear accumulation of PCNA in response to ionizing radiation. In support of a p53-independent role of MTA1 in DNA damage response, we further demonstrate that induced expression of MTA1 in p53-null cells inhibits p21(WAF1) promoter activity and p21(WAF1) binding to PCNA. Consequently, MTA1 expression in p53-null cells results in increased induction of gamma H2AX foci and DNA double strand break repair, and decreased DNA damage sensitivity following ionizing radiation treatment. These findings uncover a new target of MTA1 and the existence of an additional p53-independent role of MTA1 in DNA damage response, at least in part, by modulating the p21(WAF1)-PCNA pathway, and thus, linking two previously unconnected NuRD complex and DNA-damage response pathways.

Pubmed ID: 20071335


  • Li DQ
  • Pakala SB
  • Reddy SD
  • Ohshiro K
  • Peng SH
  • Lian Y
  • Fu SW
  • Kumar R


The Journal of biological chemistry

Publication Data

March 26, 2010

Associated Grants

  • Agency: NCI NIH HHS, Id: CA98823
  • Agency: NCI NIH HHS, Id: CA98823-S1

Mesh Terms

  • Animals
  • Antigens, Nuclear
  • Cell Line, Tumor
  • Cell Nucleus
  • Cell Proliferation
  • Cyclin-Dependent Kinase Inhibitor p21
  • Histone Deacetylases
  • Humans
  • Mice
  • Mice, Knockout
  • Oligonucleotide Array Sequence Analysis
  • Proliferating Cell Nuclear Antigen
  • Promoter Regions, Genetic
  • Repressor Proteins
  • Transcription Factors
  • Tumor Suppressor Protein p53