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Coordinated actions of actin and BAR proteins upstream of dynamin at endocytic clathrin-coated pits.

The GTPase dynamin, a key player in endocytic membrane fission, interacts with numerous proteins that regulate actin dynamics and generate/sense membrane curvature. To determine the functional relationship between these proteins and dynamin, we have analyzed endocytic intermediates that accumulate in cells that lack dynamin (derived from dynamin 1 and 2 double conditional knockout mice). In these cells, actin-nucleating proteins, actin, and BAR domain proteins accumulate at the base of arrested endocytic clathrin-coated pits, where they support the growth of dynamic long tubular necks. These results, which we show reflect the sequence of events in wild-type cells, demonstrate a concerted action of these proteins prior to, and independent of, dynamin and emphasize similarities between clathrin-mediated endocytosis in yeast and higher eukaryotes. Our data also demonstrate that the relationship between dynamin and actin is intimately connected to dynamin's endocytic role and that dynamin terminates a powerful actin- and BAR protein-dependent tubulating activity.

Pubmed ID: 20059951

Authors

  • Ferguson SM
  • Ferguson S
  • Raimondi A
  • Paradise S
  • Shen H
  • Mesaki K
  • Ferguson A
  • Destaing O
  • Ko G
  • Takasaki J
  • Cremona O
  • O' Toole E
  • De Camilli P

Journal

Developmental cell

Publication Data

December 11, 2009

Associated Grants

  • Agency: NCI NIH HHS, Id: CA46128
  • Agency: NIDA NIH HHS, Id: DA018343
  • Agency: NIDDK NIH HHS, Id: DK45735
  • Agency: Telethon, Id: GGP05141
  • Agency: NINDS NIH HHS, Id: NS36251
  • Agency: NCI NIH HHS, Id: P01 CA046128
  • Agency: NCI NIH HHS, Id: P01 CA046128-20
  • Agency: NIDA NIH HHS, Id: P30 DA018343
  • Agency: NIDA NIH HHS, Id: P30 DA018343-06
  • Agency: NIDDK NIH HHS, Id: P30 DK045735
  • Agency: NIDDK NIH HHS, Id: P30 DK045735-119008
  • Agency: NINDS NIH HHS, Id: R01 NS036251
  • Agency: NINDS NIH HHS, Id: R01 NS036251-10
  • Agency: NINDS NIH HHS, Id: R01 NS036251-11
  • Agency: NINDS NIH HHS, Id: R01 NS036251-12
  • Agency: NINDS NIH HHS, Id: R01 NS036251-13
  • Agency: NINDS NIH HHS, Id: R37 NS036251
  • Agency: NCRR NIH HHS, Id: RR-000592
  • Agency: Canadian Institutes of Health Research, Id:

Mesh Terms

  • Actins
  • Animals
  • Cell Membrane
  • Clathrin
  • Coated Pits, Cell-Membrane
  • Cytoskeleton
  • Dynamin I
  • Dynamin II
  • Endocytosis
  • Gene Knockout Techniques
  • Mice
  • Mice, Knockout