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A reprogrammable mouse strain from gene-targeted embryonic stem cells.

The derivation of induced pluripotent stem cells (iPSCs) usually involves the viral introduction of reprogramming factors into somatic cells. Here we used gene targeting to generate a mouse strain with a single copy of an inducible, polycistronic reprogramming cassette, allowing for the induction of pluripotency in various somatic cell types. As these 'reprogrammable mice' can be easily bred, they are a useful tool to study the mechanisms underlying cellular reprogramming.

Pubmed ID: 20010832


  • Stadtfeld M
  • Maherali N
  • Borkent M
  • Hochedlinger K


Nature methods

Publication Data

January 29, 2010

Associated Grants

  • Agency: NIH HHS, Id: DP2 OD003266

Mesh Terms

  • Animals
  • Blood Cells
  • Cell Dedifferentiation
  • Cell Lineage
  • Cells, Cultured
  • Cellular Reprogramming
  • Collagen
  • DNA Nucleotidyltransferases
  • Doxycycline
  • Embryonic Stem Cells
  • Fibroblasts
  • Gene Targeting
  • Induced Pluripotent Stem Cells
  • Mice
  • Mice, Mutant Strains
  • Mutagenesis, Insertional