• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Nix is a selective autophagy receptor for mitochondrial clearance.

Autophagy is the cellular homeostatic pathway that delivers large cytosolic materials for degradation in the lysosome. Recent evidence indicates that autophagy mediates selective removal of protein aggregates, organelles and microbes in cells. Yet, the specificity in targeting a particular substrate to the autophagy pathway remains poorly understood. Here, we show that the mitochondrial protein Nix is a selective autophagy receptor by binding to LC3/GABARAP proteins, ubiquitin-like modifiers that are required for the growth of autophagosomal membranes. In cultured cells, Nix recruits GABARAP-L1 to damaged mitochondria through its amino-terminal LC3-interacting region. Furthermore, ablation of the Nix:LC3/GABARAP interaction retards mitochondrial clearance in maturing murine reticulocytes. Thus, Nix functions as an autophagy receptor, which mediates mitochondrial clearance after mitochondrial damage and during erythrocyte differentiation.

Pubmed ID: 20010802

Authors

  • Novak I
  • Kirkin V
  • McEwan DG
  • Zhang J
  • Wild P
  • Rozenknop A
  • Rogov V
  • Löhr F
  • Popovic D
  • Occhipinti A
  • Reichert AS
  • Terzic J
  • Dötsch V
  • Ney PA
  • Dikic I

Journal

EMBO reports

Publication Data

January 24, 2010

Associated Grants

  • Agency: NIDDK NIH HHS, Id: DK074519
  • Agency: NCI NIH HHS, Id: P30 CA21765

Mesh Terms

  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Autophagy
  • Binding Sites
  • Blotting, Western
  • COS Cells
  • Cells, Cultured
  • Cercopithecus aethiops
  • Humans
  • Membrane Proteins
  • Mice
  • Microtubule-Associated Proteins
  • Mitochondria
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Protein Binding
  • Proto-Oncogene Proteins
  • Receptors, GABA-A
  • Reticulocytes
  • Saccharomyces cerevisiae Proteins
  • Substrate Specificity
  • Tumor Suppressor Proteins
  • Ubiquitin-Protein Ligases