• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA.

Ribosomal processing requires a series of endo- and exonucleolytic steps for the production of mature ribosomes, of which most have been described. To ensure ribosome synthesis, 3' end formation of rRNA uses multiple nucleases acting in parallel; however, a similar parallel mechanism had not been described for 5' end maturation. Here, we identify Rrp17p as a previously unidentified 5'-3' exonuclease essential for ribosome biogenesis, functioning with Rat1p in a parallel processing pathway analogous to that of 3' end formation. Rrp17p is required for efficient exonuclease digestion of the mature 5' ends of 5.8S(S) and 25S rRNAs, contains a catalytic domain close to its N terminus, and is highly conserved among higher eukaryotes, being a member of a family of exonucleases. We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export.

Pubmed ID: 20005841


  • Oeffinger M
  • Zenklusen D
  • Ferguson A
  • Wei KE
  • El Hage A
  • Tollervey D
  • Chait BT
  • Singer RH
  • Rout MP


Molecular cell

Publication Data

December 11, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM57071
  • Agency: NIGMS NIH HHS, Id: R01 GM057071
  • Agency: NIGMS NIH HHS, Id: R01 GM062427
  • Agency: NCRR NIH HHS, Id: RR00862
  • Agency: NCRR NIH HHS, Id: RR022220
  • Agency: Wellcome Trust, Id:

Mesh Terms

  • Amino Acid Sequence
  • Exonucleases
  • Exoribonucleases
  • Humans
  • Membrane Proteins
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • RNA Precursors
  • RNA Processing, Post-Transcriptional
  • RNA, Ribosomal
  • Ribosomes
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment