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Rrp17p is a eukaryotic exonuclease required for 5' end processing of Pre-60S ribosomal RNA.

Molecular cell | Dec 11, 2009

http://www.ncbi.nlm.nih.gov/pubmed/20005841

Ribosomal processing requires a series of endo- and exonucleolytic steps for the production of mature ribosomes, of which most have been described. To ensure ribosome synthesis, 3' end formation of rRNA uses multiple nucleases acting in parallel; however, a similar parallel mechanism had not been described for 5' end maturation. Here, we identify Rrp17p as a previously unidentified 5'-3' exonuclease essential for ribosome biogenesis, functioning with Rat1p in a parallel processing pathway analogous to that of 3' end formation. Rrp17p is required for efficient exonuclease digestion of the mature 5' ends of 5.8S(S) and 25S rRNAs, contains a catalytic domain close to its N terminus, and is highly conserved among higher eukaryotes, being a member of a family of exonucleases. We show that Rrp17p binds late pre-60S ribosomes, accompanying them from the nucleolus to the nuclear periphery, and provide evidence for physical and functional links between late 60S subunit processing and export.

Pubmed ID: 20005841 RIS Download

Mesh terms: Amino Acid Sequence | Exonucleases | Exoribonucleases | Humans | Membrane Proteins | Molecular Sequence Data | Protein Structure, Tertiary | RNA Precursors | RNA Processing, Post-Transcriptional | RNA, Ribosomal | Ribosomes | Saccharomyces cerevisiae Proteins | Sequence Alignment

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM57071
  • Agency: NIGMS NIH HHS, Id: R01 GM057071
  • Agency: NIGMS NIH HHS, Id: R01 GM062427
  • Agency: NCRR NIH HHS, Id: RR00862
  • Agency: NCRR NIH HHS, Id: RR022220
  • Agency: Wellcome Trust, Id:

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