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Floxin, a resource for genetically engineering mouse ESCs.

Nature methods | Jan 29, 2010

http://www.ncbi.nlm.nih.gov/pubmed/19966808

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.

Pubmed ID: 19966808 RIS Download

Mesh terms: Alleles | Animals | Attachment Sites, Microbiological | Base Sequence | Cell Line | Embryonic Stem Cells | Enhancer Elements, Genetic | Expressed Sequence Tags | Genetic Engineering | Genetic Markers | Genetic Vectors | Integrases | Mice | Mice, Transgenic | Mutation

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Associated grants

  • Agency: NIAMS NIH HHS, Id: R01 AR054396
  • Agency: NIAMS NIH HHS, Id: R01 AR054396-01A1
  • Agency: NIAMS NIH HHS, Id: R01AR054396

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