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Floxin, a resource for genetically engineering mouse ESCs.

Nature methods | 2010

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.

Pubmed ID: 19966808 RIS Download

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Associated grants

  • Agency: NIAMS NIH HHS, United States
    Id: R01 AR054396
  • Agency: NIAMS NIH HHS, United States
    Id: R01 AR054396-01A1
  • Agency: NIAMS NIH HHS, United States
    Id: R01AR054396

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Covance (tool)

RRID:SCR_001224

A contract research organization providing drug development and animal testing services. Under the name Covance Research Products Inc., based in Denver, Pennsylvania, the company also deals in the import, breeding and sale of laboratory animals. It breeds dogs, rabbits, guinea pigs, non-human primates, and pigs, and runs the largest non-human primate laboratory in Germany. (Wikipedia)

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