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Impact of genome reduction on bacterial metabolism and its regulation.

Science (New York, N.Y.) | Nov 27, 2009

http://www.ncbi.nlm.nih.gov/pubmed/19965476

To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and 13C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.

Pubmed ID: 19965476 RIS Download

Mesh terms: Adenosine Triphosphate | Bacterial Proteins | Culture Media | Energy Metabolism | Enzymes | Gene Expression Profiling | Gene Expression Regulation, Bacterial | Genome, Bacterial | Glycolysis | Metabolic Networks and Pathways | Mycoplasma pneumoniae | RNA, Bacterial | Signal Transduction | Systems Biology | Transcription, Genetic | rRNA Operon