We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Impact of genome reduction on bacterial metabolism and its regulation.

Science (New York, N.Y.) | Nov 27, 2009

To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and 13C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.

Pubmed ID: 19965476 RIS Download

Mesh terms: Adenosine Triphosphate | Bacterial Proteins | Culture Media | Energy Metabolism | Enzymes | Gene Expression Profiling | Gene Expression Regulation, Bacterial | Genome, Bacterial | Glycolysis | Metabolic Networks and Pathways | Mycoplasma pneumoniae | RNA, Bacterial | Signal Transduction | Systems Biology | Transcription, Genetic | rRNA Operon