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Impact of genome reduction on bacterial metabolism and its regulation.

To understand basic principles of bacterial metabolism organization and regulation, but also the impact of genome size, we systematically studied one of the smallest bacteria, Mycoplasma pneumoniae. A manually curated metabolic network of 189 reactions catalyzed by 129 enzymes allowed the design of a defined, minimal medium with 19 essential nutrients. More than 1300 growth curves were recorded in the presence of various nutrient concentrations. Measurements of biomass indicators, metabolites, and 13C-glucose experiments provided information on directionality, fluxes, and energetics; integration with transcription profiling enabled the global analysis of metabolic regulation. Compared with more complex bacteria, the M. pneumoniae metabolic network has a more linear topology and contains a higher fraction of multifunctional enzymes; general features such as metabolite concentrations, cellular energetics, adaptability, and global gene expression responses are similar, however.

Pubmed ID: 19965476


  • Yus E
  • Maier T
  • Michalodimitrakis K
  • van Noort V
  • Yamada T
  • Chen WH
  • Wodke JA
  • Güell M
  • Martínez S
  • Bourgeois R
  • Kühner S
  • Raineri E
  • Letunic I
  • Kalinina OV
  • Rode M
  • Herrmann R
  • Gutiérrez-Gallego R
  • Russell RB
  • Gavin AC
  • Bork P
  • Serrano L


Science (New York, N.Y.)

Publication Data

November 27, 2009

Associated Grants


Mesh Terms

  • Adenosine Triphosphate
  • Bacterial Proteins
  • Culture Media
  • Energy Metabolism
  • Enzymes
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Genome, Bacterial
  • Glycolysis
  • Metabolic Networks and Pathways
  • Mycoplasma pneumoniae
  • RNA, Bacterial
  • Signal Transduction
  • Systems Biology
  • Transcription, Genetic
  • rRNA Operon