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Distribution of RGS9-2 in neurons of the mouse striatum.

Journal of neurochemistry | Feb 20, 2010

Regulators of G protein signaling (RGS) proteins negatively modulate G protein-coupled receptor (GPCR) signaling activity by accelerating G protein hydrolysis of GTP, hastening pathway shutoff. A wealth of data from cell culture experiments using exogenously expressed proteins indicates that RGS9 and other RGS proteins have the potential to down-regulate a significant number of pathways. We have used an array of biochemical and tissue staining techniques to examine the subcellular localization and membrane binding characteristics of endogenous RGS9-2 and known binding partners in rodent striatum and tissue homogenates. A small fraction of RGS9-2 is present in the soluble cytoplasmic fraction, whereas the majority is present primarily associated with the plasma membrane and structures insoluble in non-ionic detergents that efficiently extract the vast majority of its binding partners, R7BP and G(beta5). It is specifically excluded from the cell nucleus in mouse striatal tissue. In cultured striatal neurons, RGS9-2 is found at extrasynaptic sites primarily along the dendritic shaft near the spine neck. Heterogeneity in RGS9-2 detergent solubility along with its unique subcellular localization suggests that its mechanism of membrane anchoring and localization is complex and likely involves additional proteins beside R7BP. An important nuclear function for RGS9-2 seems unlikely.

Pubmed ID: 19912469 RIS Download

Mesh terms: Animals | Cells, Cultured | Cerebral Cortex | Coculture Techniques | Corpus Striatum | Dopamine and cAMP-Regulated Phosphoprotein 32 | GTP-Binding Protein beta Subunits | Glutamate Decarboxylase | Membrane Microdomains | Mice | Mice, Inbred C57BL | Mice, Knockout | Microfilament Proteins | Nerve Tissue Proteins | Neurons | Protein Binding | RGS Proteins | Rats | Rats, Sprague-Dawley | Subcellular Fractions | Synapses | Transcription Factor TFIID

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Associated grants

  • Agency: NIDA NIH HHS, Id: R01-DA15189
  • Agency: NEI NIH HHS, Id: R01 EY007981
  • Agency: NEI NIH HHS, Id: R01 EY011900
  • Agency: NIDDK NIH HHS, Id: T32 DK007696
  • Agency: NEI NIH HHS, Id: R01-EY11900
  • Agency: NIDA NIH HHS, Id: R01 DA015189
  • Agency: NINDS NIH HHS, Id: R01 NS055339
  • Agency: NEI NIH HHS, Id: P30 EY002520
  • Agency: NIDDK NIH HHS, Id: T32- DK007696
  • Agency: NINDS NIH HHS, Id: R01-NS055339

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