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Conserved telomere maintenance component 1 interacts with STN1 and maintains chromosome ends in higher eukaryotes.

Orthologs of the yeast telomere protein Stn1 are present in plants, but other components of the Cdc13/Stn1/Ten1 (CST) complex have only been found in fungi. Here we report the identification of conserved telomere maintenance component 1 (CTC1) in plants and vertebrates. CTC1 encodes an approximately 140 kDa telomere-associated protein predicted to contain multiple OB-fold domains. Arabidopsis mutants null for CTC1 display a severe telomere deprotection phenotype accompanied by a rapid onset of developmental defects and sterility. Telomeric and subtelomeric tracts are dramatically eroded, and chromosome ends exhibit increased G overhangs, recombination, and end-to-end fusions. AtCTC1 both physically and genetically interacts with AtSTN1. Depletion of human CTC1 by RNAi triggers a DNA damage response, chromatin bridges, increased G overhangs, and sporadic telomere loss. These data indicate that CTC1 participates in telomere maintenance in diverse species and that a CST-like complex is required for telomere integrity in multicellular organisms.

Pubmed ID: 19854131

Authors

  • Surovtseva YV
  • Churikov D
  • Boltz KA
  • Song X
  • Lamb JC
  • Warrington R
  • Leehy K
  • Heacock M
  • Price CM
  • Shippen DE

Journal

Molecular cell

Publication Data

October 23, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM041803
  • Agency: NIGMS NIH HHS, Id: GM065383
  • Agency: NIGMS NIH HHS, Id: GM73169
  • Agency: NIGMS NIH HHS, Id: GM800052
  • Agency: NIGMS NIH HHS, Id: R01 GM041803
  • Agency: NIGMS NIH HHS, Id: R01 GM041803-19
  • Agency: NIGMS NIH HHS, Id: R01 GM073169
  • Agency: NIGMS NIH HHS, Id: R01 GM073169-04

Mesh Terms

  • Anaphase
  • Arabidopsis
  • Arabidopsis Proteins
  • Cell Line, Tumor
  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Plant
  • Conserved Sequence
  • Eukaryotic Cells
  • Genomic Instability
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mutation
  • Nucleic Acid Conformation
  • Protein Binding
  • Recombination, Genetic
  • Telomere
  • Telomere-Binding Proteins