Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Mrgprd-expressing polymodal nociceptive neurons innervate most known classes of substantia gelatinosa neurons.

The Mas-related G-protein-coupled receptor D (Mrgprd) marks a distinct subset of sensory neurons that transmit polymodal nociceptive information from the skin epidermis to the substantia gelatinosa (SG, lamina II) of the spinal cord. Moreover, Mrgprd-expressing (Mrgprd(+)) neurons are required for the full expression of mechanical but not thermal nociception. While such anatomical and functional specificity suggests Mrgprd(+) neurons might synapse with specific postsynaptic targets in the SG, precisely how Mrgprd(+) neurons interface with spinal circuits is currently unknown. To study circuit connectivity, we genetically targeted the light-activated ion channel Channelrhodopsin-2-Venus (ChR2-Venus) to the Mrgprd locus. In these knock-in mice, ChR2-Venus was localized to nonpeptidergic Mrgprd(+) neurons and axons, while peptidergic CGRP(+) neurons were not significantly labeled. Dissociated Mrgprd(+) DRG neurons from mice expressing one or two copies of ChR2-Venus could be activated in vitro as evidenced by light-evoked currents and action potentials. In addition, illumination of Mrgprd-ChR2-Venus(+) axon terminals in spinal cord slices evoked EPSCs in half of all SG neurons. Within this subset, Mrgprd(+) neurons were monosynaptically connected to most known classes of SG neurons, including radial, tonic central, transient central, vertical, and antenna cells. This cellular diversity ruled out the possibility that Mrgprd(+) neurons innervate a dedicated class of SG neuron. Our findings set broad constraints on the types of spinal neurons that process afferent input from Mrgprd(+) polymodal nociceptors.

Pubmed ID: 19846708 RIS Download

Mesh terms: Animals | Biophysics | Calcitonin Gene-Related Peptide | Cells, Cultured | Electric Stimulation | Excitatory Postsynaptic Potentials | Ganglia, Spinal | Green Fluorescent Proteins | In Vitro Techniques | Lectins | Membrane Potentials | Mice | Mice, Inbred C57BL | Mice, Transgenic | Nerve Net | Nociceptors | Patch-Clamp Techniques | Photic Stimulation | Receptors, G-Protein-Coupled | Rhodopsin | Sensory Receptor Cells | Substantia Gelatinosa

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NINDS NIH HHS, Id: R01 NS060725
  • Agency: NINDS NIH HHS, Id: R01 NS060725-02
  • Agency: NINDS NIH HHS, Id: R01 NS060725-01
  • Agency: NINDS NIH HHS, Id: R01 NS060725-04
  • Agency: NINDS NIH HHS, Id: P30 NS045892
  • Agency: NINDS NIH HHS, Id: R01 NS060725-03
  • Agency: NINDS NIH HHS, Id: P30NS045892
  • Agency: NINDS NIH HHS, Id: R01NS060725

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.