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Differential requirement for Gata1 DNA binding and transactivation between primitive and definitive stages of hematopoiesis in zebrafish.

Blood | Dec 10, 2009

The transcription factor Gata1 is required for the development of erythrocytes and megakaryocytes. Previous studies with a complementation rescue approach showed that the zinc finger domains are required for both primitive and definitive hematopoiesis. Here we report a novel zebrafish gata1 mutant with an N-ethyl-N-nitrosourea-induced point mutation in the C-finger (gata1(T301K)). The Gata1 protein with this mutation bound to its DNA target sequence with reduced affinity and transactivated inefficiently in a reporter assay. gata1(T301K/T301K) fish had a decreased number of erythrocytes during primitive hematopoiesis but normal adult hematopoiesis. We crossed the gata1(T301K/T301K) fish with those carrying the R339X mutation, also known as vlad tepes (vlt), which abolishes DNA binding and transactivation activities. As we reported previously, gata1(vlt/vlt) embryos were "bloodless" and died approximately 11 to 15 days after fertilization. Interestingly, the gata1(T301K/vlt) fish had nearly a complete block of primitive hematopoiesis, but they resumed hematopoiesis between 7 and 14 days after fertilization and grew to phenotypically normal fish with normal adult hematopoiesis. Our findings suggest that the impact of Gata1 on hematopoiesis correlates with its DNA-binding ability and that primitive hematopoiesis is more sensitive to reduction in Gata1 function than definitive hematopoiesis.

Pubmed ID: 19843882 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Binding Sites | DNA | Embryo, Nonmammalian | Female | Flow Cytometry | GATA1 Transcription Factor | Gene Expression Regulation, Developmental | Hematopoiesis | In Situ Hybridization | Male | Models, Molecular | Molecular Sequence Data | Mutation | Nucleic Acid Conformation | Protein Binding | Protein Structure, Tertiary | Reverse Transcriptase Polymerase Chain Reaction | Sequence Homology, Amino Acid | Transcriptional Activation | Zebrafish | Zebrafish Proteins

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Associated grants

  • Agency: Intramural NIH HHS, Id: Z01 HG000102-11
  • Agency: Intramural NIH HHS, Id: Z99 HG999999

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