• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes.

T cells develop in the thymus through positive and negative selection, which are responsible for shaping the T cell receptor (TCR) repertoire. To elucidate the molecular mechanisms involved in selection remains an area of intense interest. Here, we identified and characterized a gene product Gasp (Grb2-associating protein, also called Themis) that is critically required for positive selection. Gasp is a cytosolic protein with no known functional motifs that is expressed only in T cells, especially immature CD4/CD8 double positive (DP) thymocytes. In the absence of Gasp, differentiation of both CD4 and CD8 single positive cells in the thymus was severely inhibited, whereas all other TCR-induced events such as beta-selection, negative selection, peripheral activation, and homeostatic proliferation were unaffected. We found that Gasp constitutively associates with Grb2 via its N-terminal Src homology 3 domain, suggesting that Gasp acts as a thymocyte-specific adaptor for Grb2 or regulates Ras signaling in DP thymocytes. Collectively, we have described a gene called Gasp that is critical for positive selection.

Pubmed ID: 19805304


  • Patrick MS
  • Oda H
  • Hayakawa K
  • Sato Y
  • Eshima K
  • Kirikae T
  • Iemura S
  • Shirai M
  • Abe T
  • Natsume T
  • Sasazuki T
  • Suzuki H


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

September 22, 2009

Associated Grants


Mesh Terms

  • Animals
  • Blotting, Western
  • CD4-Positive T-Lymphocytes
  • CD8-Positive T-Lymphocytes
  • Cell Line
  • Cytosol
  • Flow Cytometry
  • Gene Expression
  • Humans
  • Immunophenotyping
  • Immunoprecipitation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Proteins
  • Receptors, Antigen, T-Cell
  • Reverse Transcriptase Polymerase Chain Reaction
  • Spleen
  • T-Lymphocytes
  • Thymus Gland