Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

MEIG1 is essential for spermiogenesis in mice.

Spermatogenesis can be divided into three stages: spermatogonial mitosis, meiosis of spermatocytes, and spermiogenesis. During spermiogenesis, spermatids undergo dramatic morphological changes including formation of a flagellum and chromosomal packaging and condensation of the nucleus into the sperm head. The genes regulating the latter processes are largely unknown. We previously discovered that a bi-functional gene, Spag16, is essential for spermatogenesis. SPAG16S, the 35 kDa, testis-specific isoform derived from the Spag16 gene, was found to bind to meiosis expressed gene 1 product (MEIG1), a protein originally thought to play a role in meiosis. We inactivated the Meig1 gene and, unexpectedly, found that Meig1 mutant male mice had no obvious defect in meiosis, but were sterile as a result of impaired spermatogenesis at the stage of elongation and condensation. Transmission electron microscopy revealed that the manchette, a microtubular organelle essential for sperm head and flagellar formation was disrupted in spermatids of MEIG1-deficient mice. We also found that MEIG1 associates with the Parkin co-regulated gene (PACRG) protein, and that testicular PACRG protein is reduced in MEIG1-deficient mice. PACRG is thought to play a key role in assembly of the axonemes/flagella and the reproductive phenotype of Pacrg-deficient mice mirrors that of the Meig1 mutant mice. Our findings reveal a critical role for the MEIG1/PARCG partnership in manchette structure and function and the control of spermiogenesis.

Pubmed ID: 19805151 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Blotting, Western | COS Cells | Cell Cycle Proteins | Cercopithecus aethiops | Genes, Essential | Green Fluorescent Proteins | Immunoprecipitation | Male | Mice | Mice, Knockout | Microscopy, Electron | Molecular Sequence Data | Nuclear Proteins | Phosphoproteins | Protein Transport | Proteins | Reverse Transcriptase Polymerase Chain Reaction | Sequence Homology, Amino Acid | Spermatids | Spermatogenesis | Testis | Two-Hybrid System Techniques

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

Associated grants

  • Agency: NICHD NIH HHS, Id: R01 HD045783
  • Agency: NICHD NIH HHS, Id: 1F31HD0G2314-01
  • Agency: NIEHS NIH HHS, Id: Z01 ES070076
  • Agency: NINDS NIH HHS, Id: P30 NS047463
  • Agency: Intramural NIH HHS, Id: R01 CA115503
  • Agency: NCI NIH HHS, Id: R01 HD037416
  • Agency: NICHD NIH HHS, Id: HD37416
  • Agency: NICHD NIH HHS, Id: CA115503
  • Agency: NCI NIH HHS, Id: 5P30NS047463
  • Agency: NINDS NIH HHS, Id: HD 045783
  • Agency: NICHD NIH HHS, Id:

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.