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UHRF1 recruits the histone acetyltransferase Tip60 and controls its expression and activity.

Tat-interactive protein, 60kDa (Tip60) is a histone acetyltransferase with specificity toward lysine 5 of histone H2A (H2AK5) and plays multiple roles in chromatin remodeling processes. Co-immunoprecipitation experiments performed on Jurkat cells, showed that Tip60 is present in the same macro-molecular complex as UHRF1 (Ubiquitin-like containing PHD and RING domain 1), DNMT1 (DNA methyltransferase 1), and HDAC1 (histone deacetylase 1). Furthermore, immunocytochemistry experiments confirmed that Tip60 co-localizes with the UHRF1/DNMT1 complex. Although down-regulation of UHRF1 by RNA interference enhanced Tip60 expression, a significant decrease of the level of acetylated H2AK5 was observed. Consistently, we have observed that down-regulation of Tip60 and DNMT1 by RNA interference, dramatically reduced the levels of acetylated H2AK5. Altogether, these results suggest that Tip60 is a novel partner of the epigenetic integration platform interplayed by UHRF1, DNMT1 and HDAC1 involved in the epigenetic code replication.

Pubmed ID: 19800870

Authors

  • Achour M
  • Fuhrmann G
  • Alhosin M
  • Rondé P
  • Chataigneau T
  • Mousli M
  • Schini-Kerth VB
  • Bronner C

Journal

Biochemical and biophysical research communications

Publication Data

December 18, 2009

Associated Grants

None

Mesh Terms

  • Acetylation
  • CCAAT-Enhancer-Binding Proteins
  • DNA (Cytosine-5-)-Methyltransferase
  • DNA Methylation
  • Epigenesis, Genetic
  • Gene Knockdown Techniques
  • Histone Acetyltransferases
  • Histones
  • Humans
  • Immunoprecipitation
  • Jurkat Cells
  • Lysine