Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Selective interaction between Trf3 and Taf3 required for early development and hematopoiesis.

http://www.ncbi.nlm.nih.gov/pubmed/19777587

In zebrafish, TATA-box-binding protein (TBP)-related factor 3, Trf3, is required for early development and initiation of hematopoiesis, and functions by promoting expression of a single target gene, mespa. Recent studies have shown that in murine muscle cells, TRF3 interacts with the TBP-associated factor TAF3. Here we investigate the role of Taf3 in zebrafish embryogenesis. We find that like Trf3-depleted zebrafish embryos, Taf3-depleted embryos exhibit multiple developmental defects and fail to undergo hematopoiesis. Both Trf3 and Taf3 are selectively bound to the mespa promoter and are required for mespa expression. Significantly, Taf3 interacts with Trf3 but not Tbp, and a Trf3 mutant that disrupts this interaction fails to support mespa transcription, early development, and hematopoiesis. Thus, a selective interaction between Trf3 and Taf3 is required for early zebrafish development and initiation of hematopoiesis. Finally, we provide evidence that TRF3 and TAF3 are also required for hematopoiesis initiation in the mouse.

Pubmed ID: 19777587 RIS Download

Mesh terms: Amino Acid Sequence | Animals | Basic Helix-Loop-Helix Transcription Factors | Cell Line | Embryo, Mammalian | Embryonic Development | Gene Expression Regulation, Developmental | Hematopoiesis | Homeodomain Proteins | Humans | Mice | Molecular Sequence Data | Promoter Regions, Genetic | Recombinant Fusion Proteins | TATA Box Binding Protein-Like Proteins | TATA-Binding Protein Associated Factors | Transcription, Genetic | Zebrafish | Zebrafish Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM33977
  • Agency: NIGMS NIH HHS, Id: R01 GM033977
  • Agency: Howard Hughes Medical Institute, Id:

ZFIN (Data, Gene Expression)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.