Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Proper timing of cytokinesis is regulated by Schizosaccharomyces pombe Etd1.

Cytokinesis must be initiated only after chromosomes have been segregated in anaphase and must be terminated once cleavage is completed. We show that the fission yeast protein Etd1 plays a central role in both of these processes. Etd1 activates the guanosine triphosphatase (GTPase) Spg1 to trigger signaling through the septum initiation network (SIN) pathway and onset of cytokinesis. Spg1 is activated in late anaphase when spindle elongation brings spindle pole body (SPB)-localized Spg1 into proximity with its activator Etd1 at cell tips, ensuring that cytokinesis is only initiated when the spindle is fully elongated. Spg1 is active at just one of the two SPBs during cytokinesis. When the actomyosin ring finishes constriction, the SIN triggers disappearance of Etd1 from the half of the cell with active Spg1, which then triggers Spg1 inactivation. Asymmetric activation of Spg1 is crucial for timely inactivation of the SIN. Together, these results suggest a mechanism whereby cell asymmetry is used to monitor cytoplasmic partitioning to turn off cytokinesis signaling.

Pubmed ID: 19736319


  • García-Cortés JC
  • McCollum D


The Journal of cell biology

Publication Data

September 7, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM058406

Mesh Terms

  • Anaphase
  • Cell Cycle Proteins
  • Cytokinesis
  • Enzyme Activation
  • GTP Phosphohydrolases
  • Gene Knockdown Techniques
  • Guanine Nucleotide Exchange Factors
  • Phenotype
  • Protein-Serine-Threonine Kinases
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
  • Signal Transduction
  • Spindle Apparatus