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Emi1 maintains genomic integrity during zebrafish embryogenesis and cooperates with p53 in tumor suppression.

A growing body of evidence indicates that early mitotic inhibitor 1 (Emi1) is essential for genomic stability, but how this function relates to embryonic development and cancer pathogenesis remains unclear. We have identified a zebrafish mutant line in which deficient emi1 gene expression results in multilineage hematopoietic defects and widespread developmental defects that are p53 independent. Cell cycle analyses of Emi1-depleted zebrafish or human cells showed chromosomal rereplication, and metaphase preparations from mutant zebrafish embryos revealed rereplicated, unsegregated chromosomes and polyploidy. Furthermore, EMI1-depleted mammalian cells relied on topoisomerase II alpha-dependent mitotic decatenation to progress through metaphase. Interestingly, the loss of a single emi1 allele in the absence of p53 enhanced the susceptibility of adult fish to neural sheath tumorigenesis. Our results cast Emi1 as a critical regulator of genomic fidelity during embryogenesis and suggest that the factor may act as a tumor suppressor.

Pubmed ID: 19704007 RIS Download

Mesh terms: Animals | Apoptosis | Cell Cycle | Cell Cycle Proteins | Cell Size | DNA Damage | Embryo, Nonmammalian | Embryonic Development | Genome | Hematopoiesis | Mutation | Myeloid Cells | Neoplasms | Phenotype | Tumor Suppressor Protein p53 | Zebrafish | Zebrafish Proteins

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Associated grants

  • Agency: NCRR NIH HHS, Id: R01 RR12589
  • Agency: NCRR NIH HHS, Id: R01 RR012589
  • Agency: NIDDK NIH HHS, Id: K01 DK69672
  • Agency: NCI NIH HHS, Id: P01 CA066996
  • Agency: NCI NIH HHS, Id: R01 CA111560
  • Agency: NIDDK NIH HHS, Id: K01 DK069672
  • Agency: NCI NIH HHS, Id: P01 CA66996-11A1
  • Agency: NCI NIH HHS, Id: R01 CA93152
  • Agency: NCI NIH HHS, Id: R01 CA093152

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