Preparing your results

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Myosin-driven peroxisome partitioning in S. cerevisiae.

In Saccharomyces cerevisiae, the class V myosin motor Myo2p propels the movement of most organelles. We recently identified Inp2p as the peroxisome-specific receptor for Myo2p. In this study, we delineate the region of Myo2p devoted to binding peroxisomes. Using mutants of Myo2p specifically impaired in peroxisome binding, we dissect cell cycle-dependent and peroxisome partitioning-dependent mechanisms of Inp2p regulation. We find that although total Inp2p levels oscillate with the cell cycle, Inp2p levels on individual peroxisomes are controlled by peroxisome inheritance, as Inp2p aberrantly accumulates and decorates all peroxisomes in mother cells when peroxisome partitioning is abolished. We also find that Inp2p is a phosphoprotein whose level of phosphorylation is coupled to the cell cycle irrespective of peroxisome positioning in the cell. Our findings demonstrate that both organelle positioning and cell cycle progression control the levels of organelle-specific receptors for molecular motors to ultimately achieve an equidistribution of compartments between mother and daughter cells.

Pubmed ID: 19687257


  • Fagarasanu A
  • Mast FD
  • Knoblach B
  • Jin Y
  • Brunner MJ
  • Logan MR
  • Glover JN
  • Eitzen GA
  • Aitchison JD
  • Weisman LS
  • Rachubinski RA


The Journal of cell biology

Publication Data

August 24, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM062261
  • Agency: NIGMS NIH HHS, Id: R01-GM62261
  • Agency: NIGMS NIH HHS, Id: R01-GM75152

Mesh Terms

  • Cell Cycle
  • Membrane Proteins
  • Mitochondria
  • Models, Molecular
  • Molecular Structure
  • Myosin Heavy Chains
  • Myosin Type V
  • Peroxisomes
  • Point Mutation
  • Protein Processing, Post-Translational
  • Protein Structure, Tertiary
  • Receptors, Cytoplasmic and Nuclear
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Two-Hybrid System Techniques
  • Vacuoles