We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1.

Science (New York, N.Y.) | Sep 4, 2009

Posttranslational modifications play key roles in regulating chromatin plasticity. Although various chromatin-remodeling enzymes have been described that respond to specific histone modifications, little is known about the role of poly[adenosine 5'-diphosphate (ADP)-ribose] in chromatin remodeling. Here, we identify a chromatin-remodeling enzyme, ALC1 (Amplified in Liver Cancer 1, also known as CHD1L), that interacts with poly(ADP-ribose) and catalyzes PARP1-stimulated nucleosome sliding. Our results define ALC1 as a DNA damage-response protein whose role in this process is sustained by its association with known DNA repair factors and its rapid poly(ADP-ribose)-dependent recruitment to DNA damage sites. Furthermore, we show that depletion or overexpression of ALC1 results in sensitivity to DNA-damaging agents. Collectively, these results provide new insights into the mechanisms by which poly(ADP-ribose) regulates DNA repair.

Pubmed ID: 19661379 RIS Download

Mesh terms: Adenosine Triphosphatases | Adenosine Triphosphate | Cell Line | Chromatin | Chromatin Assembly and Disassembly | DNA Damage | DNA Helicases | DNA Repair | DNA-Binding Proteins | Humans | Hydrogen Peroxide | Immunoprecipitation | Kinetics | Mutant Proteins | Nucleosomes | Phleomycins | Poly (ADP-Ribose) Polymerase-1 | Poly Adenosine Diphosphate Ribose | Poly(ADP-ribose) Polymerase Inhibitors | Poly(ADP-ribose) Polymerases | Protein Structure, Tertiary | Radiation, Ionizing | Recombinant Proteins

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Cancer Research UK, Id: 11224
  • Agency: Wellcome Trust, Id: 064414
  • Agency: Biotechnology and Biological Sciences Research Council, Id: A3549
  • Agency: Department of Health, Id: A5290
  • Agency: Cancer Research UK, Id:
  • Agency: Cancer Research UK, Id:
  • Agency: Cancer Research UK, Id:

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.