We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Telomerase modulates Wnt signalling by association with target gene chromatin.

Nature | Jul 2, 2009

Stem cells are controlled, in part, by genetic pathways frequently dysregulated during human tumorigenesis. Either stimulation of Wnt/beta-catenin signalling or overexpression of telomerase is sufficient to activate quiescent epidermal stem cells in vivo, although the mechanisms by which telomerase exerts these effects are not understood. Here we show that telomerase directly modulates Wnt/beta-catenin signalling by serving as a cofactor in a beta-catenin transcriptional complex. The telomerase protein component TERT (telomerase reverse transcriptase) interacts with BRG1 (also called SMARCA4), a SWI/SNF-related chromatin remodelling protein, and activates Wnt-dependent reporters in cultured cells and in vivo. TERT serves an essential role in formation of the anterior-posterior axis in Xenopus laevis embryos, and this defect in Wnt signalling manifests as homeotic transformations in the vertebrae of Tert(-/-) mice. Chromatin immunoprecipitation of the endogenous TERT protein from mouse gastrointestinal tract shows that TERT physically occupies gene promoters of Wnt-dependent genes. These data reveal an unanticipated role for telomerase as a transcriptional modulator of the Wnt/beta-catenin signalling pathway.

Pubmed ID: 19571879 RIS Download

Mesh terms: Animals | Cell Line | Choristoma | Chromatin | DNA Helicases | Genes, Reporter | HeLa Cells | Humans | Intestine, Small | Mice | Nuclear Proteins | Oocytes | Plasmids | Promoter Regions, Genetic | Signal Transduction | Somites | Telomerase | Transcription Factors | Wnt Proteins | Wnt3 Protein | Xenopus laevis | beta Catenin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA125453
  • Agency: NCI NIH HHS, Id: CA111691
  • Agency: NCI NIH HHS, Id: R01 CA111691
  • Agency: NCI NIH HHS, Id: R01 CA125453
  • Agency: NCI NIH HHS, Id: P30 CA124435

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.