Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Nature methods | Jul 30, 2009

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Pubmed ID: 19525957 RIS Download

Mesh terms: Agouti Signaling Protein | Animals | Base Sequence | Cell Line | Crosses, Genetic | DNA Primers | Embryonic Stem Cells | Female | Gene Targeting | Genetic Techniques | Germ-Line Mutation | Hair Color | Male | Mice | Mice, Inbred C57BL | Mice, Knockout | Mice, Mutant Strains | Mice, Transgenic | Polymorphism, Single Nucleotide | Pregnancy | Transplantation Chimera

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: Wellcome Trust, Id: WT077187
  • Agency: PHS HHS, Id: U01-42430
  • Agency: NIH HHS, Id: UM1 OD023221
  • Agency: NHGRI NIH HHS, Id: U01 HG004080
  • Agency: Wellcome Trust, Id: 077188
  • Agency: NHGRI NIH HHS, Id: U01-HG004080

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.