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Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Pubmed ID: 19525957


  • Pettitt SJ
  • Liang Q
  • Rairdan XY
  • Moran JL
  • Prosser HM
  • Beier DR
  • Lloyd KC
  • Bradley A
  • Skarnes WC


Nature methods

Publication Data

July 30, 2009

Associated Grants

  • Agency: Wellcome Trust, Id: 077188
  • Agency: PHS HHS, Id: U01-42430
  • Agency: NHGRI NIH HHS, Id: U01-HG004080
  • Agency: Wellcome Trust, Id: WT077187

Mesh Terms

  • Agouti Signaling Protein
  • Animals
  • Base Sequence
  • Cell Line
  • Crosses, Genetic
  • DNA Primers
  • Embryonic Stem Cells
  • Female
  • Gene Targeting
  • Genetic Techniques
  • Germ-Line Mutation
  • Hair Color
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Polymorphism, Single Nucleotide
  • Pregnancy
  • Transplantation Chimera