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Agouti C57BL/6N embryonic stem cells for mouse genetic resources.

Nature methods | Jul 30, 2009

We report the characterization of a highly germline competent C57BL/6N mouse embryonic stem cell line, JM8. To simplify breeding schemes, the dominant agouti coat color gene was restored in JM8 cells by targeted repair of the C57BL/6 nonagouti mutation. These cells provide a robust foundation for large-scale mouse knockout programs that aim to provide a public resource of targeted mutations in the C57BL/6 genetic background.

Pubmed ID: 19525957 RIS Download

Mesh terms: Agouti Signaling Protein | Animals | Base Sequence | Cell Line | Crosses, Genetic | DNA Primers | Embryonic Stem Cells | Female | Gene Targeting | Genetic Techniques | Germ-Line Mutation | Hair Color | Male | Mice | Mice, Inbred C57BL | Mice, Knockout | Mice, Mutant Strains | Mice, Transgenic | Polymorphism, Single Nucleotide | Pregnancy | Transplantation Chimera

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Associated grants

  • Agency: Wellcome Trust, Id: WT077187
  • Agency: PHS HHS, Id: U01-42430
  • Agency: NIH HHS, Id: UM1 OD023221
  • Agency: NHGRI NIH HHS, Id: U01 HG004080
  • Agency: Wellcome Trust, Id: WT077188
  • Agency: NHGRI NIH HHS, Id: U01-HG004080

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