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An rtt109-independent role for vps75 in transcription-associated nucleosome dynamics.


The histone chaperone Vps75 forms a complex with, and stimulates the activity of, the histone acetyltransferase Rtt109. However, Vps75 can also be isolated on its own and might therefore possess Rtt109-independent functions. Analysis of epistatic miniarray profiles showed that VPS75 genetically interacts with factors involved in transcription regulation whereas RTT109 clusters with genes linked to DNA replication/repair. Additional genetic and biochemical experiments revealed a close relationship between Vps75 and RNA polymerase II. Furthermore, Vps75 is recruited to activated genes in an Rtt109-independent manner, and its genome-wide association with genes correlates with transcription rate. Expression microarray analysis identified a number of genes whose normal expression depends on VPS75. Interestingly, histone H2B dynamics at some of these genes are consistent with a role for Vps75 in histone H2A/H2B eviction/deposition during transcription. Indeed, reconstitution of nucleosome disassembly using the ATP-dependent chromatin remodeler Rsc and Vps75 revealed that these proteins can cooperate to remove H2A/H2B dimers from nucleosomes. These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109.

Pubmed ID: 19470761


  • Selth LA
  • Lorch Y
  • Ocampo-Hafalla MT
  • Mitter R
  • Shales M
  • Krogan NJ
  • Kornberg RD
  • Svejstrup JQ


Molecular and cellular biology

Publication Data

August 14, 2009

Associated Grants

  • Agency: NIGMS NIH HHS, Id: R01 GM084448

Mesh Terms

  • Acetylation
  • Binding Sites
  • Chromatin Immunoprecipitation
  • Cluster Analysis
  • Gene Expression Profiling
  • Genome, Fungal
  • Histone Acetyltransferases
  • Histones
  • Lysine
  • Molecular Chaperones
  • Mutation
  • Nucleosomes
  • Oligonucleotide Array Sequence Analysis
  • Protein Binding
  • RNA Polymerase II
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription, Genetic