Genome-wide analysis of chromatin regulation by cocaine reveals a role for sirtuins.
Changes in gene expression contribute to the long-lasting regulation of the brain's reward circuitry seen in drug addiction; however, the specific genes regulated and the transcriptional mechanisms underlying such regulation remain poorly understood. Here, we used chromatin immunoprecipitation coupled with promoter microarray analysis to characterize genome-wide chromatin changes in the mouse nucleus accumbens, a crucial brain reward region, after repeated cocaine administration. Our findings reveal several interesting principles of gene regulation by cocaine and of the role of DeltaFosB and CREB, two prominent cocaine-induced transcription factors, in this brain region. The findings also provide comprehensive insight into the molecular pathways regulated by cocaine-including a new role for sirtuins (Sirt1 and Sirt2)-which are induced in the nucleus accumbens by cocaine and, in turn, dramatically enhance the behavioral effects of the drug.
Pubmed ID: 19447090 RIS Download
Animals | Chromatin | Cocaine | Cyclic AMP Response Element-Binding Protein | Dopamine Uptake Inhibitors | Feedback, Physiological | Gene Expression Regulation | Genome-Wide Association Study | Male | Mice | Mice, Inbred C57BL | Nucleus Accumbens | Oligonucleotide Array Sequence Analysis | Promoter Regions, Genetic | Proto-Oncogene Proteins c-fos | Reinforcement (Psychology) | Signal Transduction | Sirtuins