We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Telomere recombination requires the MUS81 endonuclease.

Nature cell biology | Jun 2, 2009

Telomerase-negative cancer cells maintain their telomeres through the alternative lengthening of telomeres (ALT) pathway. Although a growing body of evidence demonstrates that the ALT mechanism is a post-replicative telomere recombination process, molecular details of this pathway are largely unknown. Here we demonstrate that MUS81, a DNA structure specific recombination endonuclease, has a key role in the maintenance of telomeres in human ALT cells. We find that MUS81 specifically localizes to ALT-associated promyelocytic leukaemia (PML) nuclear bodies (APBs) and associates with telomeric DNA in ALT cells, which is enriched during the G2 phase of the cell cycle. Depletion of MUS81 results in the reduction of ALT-specific telomere recombination and leads to proliferation arrest of ALT cells. In addition, the endonuclease activity of MUS81 is required for recombination-based ALT cell survival, and the interaction of MUS81 with the telomeric repeat-binding factor TRF2 regulates this enzymatic activity, thereby maintaining telomere recombination. Thus, our results suggest that MUS81 is involved in the maintenance of ALT cell survival at least in part by homologous recombination of telomeres.

Pubmed ID: 19363487 RIS Download

Mesh terms: Animals | Biocatalysis | Cell Cycle | Cell Line, Tumor | Cell Nucleolus | Cell Nucleus | Cell Proliferation | Cell Survival | Chromatin Immunoprecipitation | DNA-Binding Proteins | Endodeoxyribonucleases | Endonucleases | G2 Phase | Humans | Mice | Protein Binding | RNA Interference | Recombination, Genetic | Sister Chromatid Exchange | Telomerase | Telomere | Telomeric Repeat Binding Protein 2

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA10445
  • Agency: NCI NIH HHS, Id: R01 CA123232-02
  • Agency: NCI NIH HHS, Id: CA123232
  • Agency: NCI NIH HHS, Id: R01 CA123232
  • Agency: Intramural NIH HHS, Id: Z01 BC005795-13
  • Agency: NCI NIH HHS, Id: P01 CA104457-040002
  • Agency: NINDS NIH HHS, Id: R01 NS034746-06S1
  • Agency: NCI NIH HHS, Id: P01 CA104457-050002
  • Agency: NCI NIH HHS, Id: P01 CA104457
  • Agency: NCI NIH HHS, Id: R01 CA129537
  • Agency: NINDS NIH HHS, Id: R01 NS034746

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.