• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

The PcG protein hPc2 interacts with the N-terminus of histone demethylase JARID1B and acts as a transcriptional co-repressor.

JARID1B (jumonji AT rich interactive domain 1B) is a large nuclear protein that is highly expressed in breast cancers and is proposed to function as a repressor of gene expression. In this paper, a phage display screen using the N-terminus of JARID1B as bait identified one of the JARID1B interacting proteins, namely PcG protein (Polycomb group) hPc2. We demonstrated that the C-terminal region, including the COOH box, was required for the interaction with the N-terminus of JARID1B. In a reporter assay system, co-expression of JARID1B with hPc2 significantly enhanced the transcriptional repression. These results support a role for hPc2 acting as a transcriptional co-repressor.

Pubmed ID: 19336002

Authors

  • Zhou W
  • Chen H
  • Zhang L

Journal

BMB reports

Publication Data

March 31, 2009

Associated Grants

None

Mesh Terms

  • Cell Line, Tumor
  • DNA-Binding Proteins
  • Humans
  • Jumonji Domain-Containing Histone Demethylases
  • Nuclear Proteins
  • Oxidoreductases, N-Demethylating
  • Peptide Library
  • Polycomb-Group Proteins
  • Protein Binding
  • Repressor Proteins
  • Transcription, Genetic
  • Ubiquitin-Protein Ligases